© Gunnar Wrobel & Felix Kokocinski, DKFZ , 2003 - 2012
Basic parameters
eg APOM
eg ENSG00000111964
eg ENSMUST00000025846
mM
nM
°C
nucleotides
Specific AutoPrime parameters
nucleotides
Extended Primer3 settings
nucleotides
nucleotides
°C
°C
°C
%
%
nucleotides
nucleotides
nucl.
°C
°C
°C
Internal oligo options
nM
nucleotides
nucleotides
nucleotides
°C
°C
°C
%
%
nucleotides
AutoPrime allows to rapidly design primers for real-time PCR measurement of eukaryotic expression. Such primers are usually selected so that they will amplify cDNA generated from mRNA but will not yield a product on the genomic DNA. This is achieved by taking advantage of the eucaryotic splicing system for mRNA. Either primer pairs are selected so that one of the primers matches the exon-exon border sequence that is not present in the genomic sequence or the pair is designed by placing each primer in a different exon so that a genomic product would include a long intronic sequence. AutoPrime provides these capabilities on top of the primer3 primer-design software.

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